Publikationer

Direct measurements of mRNA translation kinetics in living cells

Metelev, Mikhail; Lundin, Erik; Volkov, Ivan; Gynna, Arvid H. et al.

Ingår i Nature Communications, 2022.

DOI för Direct measurements of mRNA translation kinetics in living cells Ladda ner fulltext (pdf) av Direct measurements of mRNA translation kinetics in living cells

Sequence specificity in DNA binding is mainly governed by association

Marklund, Emil; Mao, Guanzhong; Yuan, Jinwen; Zikrin, Spartak et al.

Ingår i Science, s. 442-445, 2022.

DOI för Sequence specificity in DNA binding is mainly governed by association Ladda ner fulltext (pdf) av Sequence specificity in DNA binding is mainly governed by association

A unique histone 3 lysine 14 chromatin signature underlies tissue-specific gene regulation

Regadas, Isabel; Dahlberg, Olle; Vaid, Roshan; Ho, Oanh et al.

Ingår i Molecular Cell, s. 1766-1780.e10, 2021.

DOI för A unique histone 3 lysine 14 chromatin signature underlies tissue-specific gene regulation Ladda ner fulltext (pdf) av A unique histone 3 lysine 14 chromatin signature underlies tissue-specific gene regulation

Defective ALC1 nucleosome remodeling confers PARPi sensitization and synthetic lethality with HRD

Hewitt, Graeme; Borel, Valerie; Segura-Bayona, Sandra; Takaki, Tohru et al.

Ingår i Molecular Cell, s. 767-783.e11, 2021.

DOI för Defective ALC1 nucleosome remodeling confers PARPi sensitization and synthetic lethality with HRD Ladda ner fulltext (pdf) av Defective ALC1 nucleosome remodeling confers PARPi sensitization and synthetic lethality with HRD

Direct measurements of erythromycin’s effect on protein synthesis kinetics in living bacterial cells

Seefeldt, A. Carolin; Aguirre Rivera, Javier; Johansson, Magnus

Ingår i Journal of Molecular Biology, 2021.

DOI för Direct measurements of erythromycin’s effect on protein synthesis kinetics in living bacterial cells Ladda ner fulltext (pdf) av Direct measurements of erythromycin’s effect on protein synthesis kinetics in living bacterial cells

Imaging-based screens of pool-synthesized cell libraries

Elf, Johan; Lawson, Michael

Ingår i Nature Methods, s. 358-365, 2021.

DOI för Imaging-based screens of pool-synthesized cell libraries

More Than Just Letters and Chemistry: Genomics Goes Mechanics

Deindl, Sebastian; Elf, Johan

Ingår i TIBS -Trends in Biochemical Sciences. Regular ed., s. 431-432, 2021.

DOI för More Than Just Letters and Chemistry: Genomics Goes Mechanics

Paladin is a phosphoinositide phosphatase regulating endosomal VEGFR2 signalling and angiogenesis

Nitzsche, Anja; Pietilä, Riikka; Love, Dominic T.; Testini, Chiara et al.

Ingår i EMBO Reports, 2021.

DOI för Paladin is a phosphoinositide phosphatase regulating endosomal VEGFR2 signalling and angiogenesis Ladda ner fulltext (pdf) av Paladin is a phosphoinositide phosphatase regulating endosomal VEGFR2 signalling and angiogenesis

Real-time measurements of aminoglycoside effects on protein synthesis in live cells

Aguirre Rivera, Javier; Larsson, Jimmy; Volkov, Ivan; Seefeldt, A. Carolin et al.

Ingår i Proceedings of the National Academy of Sciences of the United States of America, 2021.

DOI för Real-time measurements of aminoglycoside effects on protein synthesis in live cells Ladda ner fulltext (pdf) av Real-time measurements of aminoglycoside effects on protein synthesis in live cells

RecA finds homologous DNA by reduced dimensionality search

Wiktor, Jakub; Gynnå, Arvid H.; Leroy, Prune; Larsson, Jimmy et al.

Ingår i Nature, s. 426-429, 2021.

DOI för RecA finds homologous DNA by reduced dimensionality search Ladda ner fulltext (pdf) av RecA finds homologous DNA by reduced dimensionality search

Reconstructing the Evolutionary History of a Highly Conserved Operon Cluster in Gammaproteobacteria and Bacilli

Brandis, Gerrit

Ingår i Genome Biology and Evolution, 2021.

DOI för Reconstructing the Evolutionary History of a Highly Conserved Operon Cluster in Gammaproteobacteria and Bacilli Ladda ner fulltext (pdf) av Reconstructing the Evolutionary History of a Highly Conserved Operon Cluster in Gammaproteobacteria and Bacilli

Structure and dynamics of the chromatin remodeler ALC1 bound to a PARylated nucleosome

Bacic, Luka; Gaullier, Guillaume; Sabantsev, Anton; Lehmann, Laura C. et al.

Ingår i eLIFE, 2021.

DOI för Structure and dynamics of the chromatin remodeler ALC1 bound to a PARylated nucleosome

The highly dynamic nature of bacterial heteroresistance impairs its clinical detection

Pereira, Cátia; Larsson, Jimmy; Hjort, Karin; Elf, Johan et al.

Ingår i Communications Biology, 2021.

DOI för The highly dynamic nature of bacterial heteroresistance impairs its clinical detection Ladda ner fulltext (pdf) av The highly dynamic nature of bacterial heteroresistance impairs its clinical detection

DNA surface exploration and operator bypassing during target search

Marklund, Emil; van Oosten, Brad; Mao, Guanzhong; Amselem, Elias et al.

Ingår i Nature, s. 858-+, 2020.

DOI för DNA surface exploration and operator bypassing during target search Ladda ner fulltext (pdf) av DNA surface exploration and operator bypassing during target search

Mechanistic Insights into Regulation of the ALC1 Remodeler by the Nucleosome Acidic Patch

Lehmann, Laura C.; Bacic, Luka; Hewitt, Graeme; Brackmann, Klaus et al.

Ingår i Cell reports, 2020.

DOI för Mechanistic Insights into Regulation of the ALC1 Remodeler by the Nucleosome Acidic Patch Ladda ner fulltext (pdf) av Mechanistic Insights into Regulation of the ALC1 Remodeler by the Nucleosome Acidic Patch

Ornithine capture by a translating ribosome controls bacterial polyamine synthesis

Herrero del Valle, Alba; Seip, Britta; Cervera-Marzal, Iñaki; Sacheau, Guénaël et al.

Ingår i Nature Microbiology, s. 554-561, 2020.

DOI för Ornithine capture by a translating ribosome controls bacterial polyamine synthesis

Rationally designed Spot 42 RNAs with an inhibition/toxicity profile advantageous for engineering E. coli

Vogel, Carolin; Gynnå, Arvid H.; Yuan, Jinwen; Bao, Letian et al.

Ingår i ENGINEERING REPORTS, 2020.

DOI för Rationally designed Spot 42 RNAs with an inhibition/toxicity profile advantageous for engineering E. coli Ladda ner fulltext (pdf) av Rationally designed Spot 42 RNAs with an inhibition/toxicity profile advantageous for engineering E. coli

Recent advances in single-molecule fluorescence microscopy render structural biology dynamic

Bacic, Luka; Sabantsev, Anton; Deindl, Sebastian

Ingår i Current opinion in structural biology, s. 61-68, 2020.

DOI för Recent advances in single-molecule fluorescence microscopy render structural biology dynamic Ladda ner fulltext (pdf) av Recent advances in single-molecule fluorescence microscopy render structural biology dynamic

RHS-elements function as type II toxin-antitoxin modules that regulate intra-macrophage replication of Salmonella Typhimurium

Stårsta, Magnus; Hammarlöf, Disa L.; Wäneskog, Marcus; Schlegel, Susan et al.

Ingår i PLoS Genetics, 2020.

DOI för RHS-elements function as type II toxin-antitoxin modules that regulate intra-macrophage replication of Salmonella Typhimurium Ladda ner fulltext (pdf) av RHS-elements function as type II toxin-antitoxin modules that regulate intra-macrophage replication of Salmonella Typhimurium

Time-resolved imaging-based CRISPRi screening

Camsund, Daniel; Lawson, Michael J.; Larsson, Jimmy; Jones, Daniel et al.

Ingår i Nature Methods, s. 86-92, 2020.

DOI för Time-resolved imaging-based CRISPRi screening

Curvature sensing by cardiolipin in simulated buckled membranes

Elias-Wolff, Federico; Lindén, Martin; Lyubartsev, Alexander P.; Brandt, Erik G.

Ingår i Soft Matter, s. 792-802, 2019.

DOI för Curvature sensing by cardiolipin in simulated buckled membranes Ladda ner fulltext (pdf) av Curvature sensing by cardiolipin in simulated buckled membranes

Direct observation of coordinated DNA movements on the nucleosome during chromatin remodelling

Sabantsev, Anton; Levendosky, Robert F.; Zhuang, Xiaowei; Bowman, Gregory D. et al.

Ingår i Nature Communications, 2019.

DOI för Direct observation of coordinated DNA movements on the nucleosome during chromatin remodelling Ladda ner fulltext (pdf) av Direct observation of coordinated DNA movements on the nucleosome during chromatin remodelling

Efficient in vivo synthesis of lasso peptide pseudomycoidin proceeds in the absence of both the leader and the leader peptidase

Zyubko, Tatyana; Serebryakova, Marina; Andreeva, Julia; Metelev, Mikhail et al.

Ingår i Chemical Science, s. 9699-9707, 2019.

DOI för Efficient in vivo synthesis of lasso peptide pseudomycoidin proceeds in the absence of both the leader and the leader peptidase Ladda ner fulltext (pdf) av Efficient in vivo synthesis of lasso peptide pseudomycoidin proceeds in the absence of both the leader and the leader peptidase

Remodeling the genome with DNA twists

Bowman, Gregory D.; Deindl, Sebastian

Ingår i Science, s. 35-36, 2019.

DOI för Remodeling the genome with DNA twists

Rotational and Translational Diffusion of Proteins as a Function of Concentration

Bashardanesh, Zahedeh; Elf, Johan; Zhang, Haiyang; Van der Spoel, David

Ingår i ACS Omega, s. 20654-20664, 2019.

DOI för Rotational and Translational Diffusion of Proteins as a Function of Concentration Ladda ner fulltext (pdf) av Rotational and Translational Diffusion of Proteins as a Function of Concentration

Single-Molecule Tracking Approaches to Protein Synthesis Kinetics in Living Cells

Volkov, Ivan; Johansson, Magnus

Ingår i Biochemistry, s. 7-14, 2019.

DOI för Single-Molecule Tracking Approaches to Protein Synthesis Kinetics in Living Cells Ladda ner fulltext (pdf) av Single-Molecule Tracking Approaches to Protein Synthesis Kinetics in Living Cells

Structure of ribosome-bound azole-modified peptide phazolicin rationalizes its species-specific mode of bacterial translation inhibition

Travin, Dmitrii Y.; Watson, Zoe L.; Metelev, Mikhail; Ward, Fred R. et al.

Ingår i Nature Communications, 2019.

DOI för Structure of ribosome-bound azole-modified peptide phazolicin rationalizes its species-specific mode of bacterial translation inhibition Ladda ner fulltext (pdf) av Structure of ribosome-bound azole-modified peptide phazolicin rationalizes its species-specific mode of bacterial translation inhibition

The ribosomal protein S1-dependent standby site in tisB mRNA consists of a single-stranded region and a 5 ' structure element

Romilly, Cedric; Deindl, Sebastian; Wagner, Gerhart E. H.

Ingår i Proceedings of the National Academy of Sciences of the United States of America, s. 15901-15906, 2019.

DOI för The ribosomal protein S1-dependent standby site in tisB mRNA consists of a single-stranded region and a 5 ' structure element Ladda ner fulltext (pdf) av The ribosomal protein S1-dependent standby site in tisB mRNA consists of a single-stranded region and a 5 ' structure element

Tracking of single tRNAs for translation kinetics measurements in chloramphenicol treated bacteria

Volkov, Ivan; Seefeldt, A. Carolin; Johansson, Magnus

Ingår i Methods, s. 23-30, 2019.

DOI för Tracking of single tRNAs for translation kinetics measurements in chloramphenicol treated bacteria Ladda ner fulltext (pdf) av Tracking of single tRNAs for translation kinetics measurements in chloramphenicol treated bacteria

Transcription and translation contribute to gene locus relocation to the nucleoid periphery in E. coli

Yang, Sora; Kim, Seunghyeon; Kim, Dong-Kyun; An, Hyeong Jeon et al.

Ingår i Nature Communications, 2019.

DOI för Transcription and translation contribute to gene locus relocation to the nucleoid periphery in E. coli Ladda ner fulltext (pdf) av Transcription and translation contribute to gene locus relocation to the nucleoid periphery in E. coli

An early peak in ion channel research

Lindén, Martin

Ingår i Nature Physics, s. 105-105, 2018.

DOI för An early peak in ion channel research

Biosynthesis of Translation Inhibitor Klebsazolicin Proceeds through Heterocyclization and N-Terminal Amidine Formation Catalyzed by a Single YcaO Enzyme

Travin, Dmitrii Y.; Metelev, Mikhail; Serebryakova, Marina; Komarova, Ekaterina S. et al.

Ingår i Journal of the American Chemical Society, s. 5625-5633, 2018.

DOI för Biosynthesis of Translation Inhibitor Klebsazolicin Proceeds through Heterocyclization and N-Terminal Amidine Formation Catalyzed by a Single YcaO Enzyme

Computing Curvature Sensitivity of Biomolecules in Membranes by Simulated Buckling

Elias-Wolff, Federico; Lindén, Martin; Lyubartsev, Alexander P.; Brandt, Erik G.

Ingår i Journal of Chemical Theory and Computation, s. 1643-1655, 2018.

DOI för Computing Curvature Sensitivity of Biomolecules in Membranes by Simulated Buckling

Contact-dependent growth inhibition induces high levels of antibiotic-tolerant persister cells in clonal bacterial populations

Ghosh, Anirban; Baltekin, Özden; Wäneskog, Marcus; Elkhalifa, Dina et al.

Ingår i EMBO Journal, 2018.

DOI för Contact-dependent growth inhibition induces high levels of antibiotic-tolerant persister cells in clonal bacterial populations

Engineering a palette of eukaryotic chromoproteins for bacterial synthetic biology

Liljeruhm, Josefine; Funk, Saskia K.; Tietscher, Sandra; Edlund, Anders D. et al.

Ingår i Journal of Biological Engineering, 2018.

DOI för Engineering a palette of eukaryotic chromoproteins for bacterial synthetic biology Ladda ner fulltext (pdf) av Engineering a palette of eukaryotic chromoproteins for bacterial synthetic biology

Evolution of high-level resistance during low-level antibiotic exposure

Wistrand-Yuen, Erik; Knopp, Michael; Hjort, Karin; Koskiniemi, Sanna et al.

Ingår i Nature Communications, 2018.

DOI för Evolution of high-level resistance during low-level antibiotic exposure Ladda ner fulltext (pdf) av Evolution of high-level resistance during low-level antibiotic exposure

KN Direct phenotypic antimicrobial susceptibility testing under 30 minutes: Dynamics of single cell response revealed in automated microscopy with microfluidics

Baltekin, Özden

Ingår i Journal of Veterinary Pharmacology and Therapeutics, s. 17-17, 2018.

DOI för KN Direct phenotypic antimicrobial susceptibility testing under 30 minutes: Dynamics of single cell response revealed in automated microscopy with microfluidics

Measuring cis-regulatory energetics in living cells using allelic manifolds

Forcier, Talitha L.; Ayaz, Andalus; Gill, Manraj S.; Jones, Daniel et al.

Ingår i eLIFE, 2018.

DOI för Measuring cis-regulatory energetics in living cells using allelic manifolds Ladda ner fulltext (pdf) av Measuring cis-regulatory energetics in living cells using allelic manifolds

New enzymatic and mass spectrometric methodology for the selective investigation of gut microbiota-derived metabolites

Ballet, Caroline; Correia, Mario S. P.; Conway, Louis P.; Locher, Theresa L. et al.

Ingår i Chemical Science, s. 6233-6239, 2018.

DOI för New enzymatic and mass spectrometric methodology for the selective investigation of gut microbiota-derived metabolites Ladda ner fulltext (pdf) av New enzymatic and mass spectrometric methodology for the selective investigation of gut microbiota-derived metabolites

Structure-guided approach to site-specific fluorophore labeling of the lac repressor LacI

Kipper, Kalle; Eremina, Nadja; Marklund, Emil; Tubasum, Sumera et al.

Ingår i PLOS ONE, 2018.

DOI för Structure-guided approach to site-specific fluorophore labeling of the lac repressor LacI Ladda ner fulltext (pdf) av Structure-guided approach to site-specific fluorophore labeling of the lac repressor LacI

Systematic approach for dissecting the molecular mechanisms of transcriptional regulation in bacteria

Belliveau, Nathan M.; Barnes, Stephanie L.; Ireland, William T.; Jones, Daniel L. et al.

Ingår i Proceedings of the National Academy of Sciences of the United States of America, s. E4796-E4805, 2018.

DOI för Systematic approach for dissecting the molecular mechanisms of transcriptional regulation in bacteria

tRNA tracking for direct measurements of protein synthesis kinetics in live cells

Volkov, Ivan; Lindén, Martin; Aguirre, Javier; Ieong, Ka-Weng et al.

Ingår i Nature Chemical Biology, s. 618-626, 2018.

DOI för tRNA tracking for direct measurements of protein synthesis kinetics in live cells

Variational Algorithms for Analyzing Noisy Multistate Diffusion Trajectories

Lindén, Martin; Elf, Johan

Ingår i Biophysical Journal, s. 276-282, 2018.

DOI för Variational Algorithms for Analyzing Noisy Multistate Diffusion Trajectories

Antibiotic susceptibility testing in less than 30 min using direct single-cell imaging

Baltekin, Özden; Boucharin, Alexis; Tano, Eva; Andersson, Dan I et al.

Ingår i Proceedings of the National Academy of Sciences of the United States of America, s. 9170-9175, 2017.

DOI för Antibiotic susceptibility testing in less than 30 min using direct single-cell imaging

Application of Noncanonical Amino Acids for Protein Labeling in a Genomically Recoded Escherichia coli

Kipper, Kalle; Lundius, Ebba G.; Ćurić, Vladimir; Nikic, Ivana et al.

Ingår i ACS Photonics, s. 233-255, 2017.

DOI för Application of Noncanonical Amino Acids for Protein Labeling in a Genomically Recoded Escherichia coli

Development of a novel therapeutic vaccine carrier that sustains high antibody titers against several targets simultaneously

Saupe, Falk; Reichel, Matthias; Huijbers, Elisabeth J. M.; Femel, Julia et al.

Ingår i The FASEB Journal, s. 1204-1214, 2017.

DOI för Development of a novel therapeutic vaccine carrier that sustains high antibody titers against several targets simultaneously

How Will Kinetics and Thermodynamics Inform Our Future Efforts to Understand and Build Biological Systems?

Doudna, Jennifer; Bar-Ziv, Roy; Elf, Johan; Noireaux, Vincent et al.

Ingår i CELL SYSTEMS, s. 144-146, 2017.

In situ genotyping of a pooled strain library after characterizing complex phenotypes

Lawson, Michael J.; Camsund, Daniel; Larsson, Jimmy; Baltekin, Özden et al.

Ingår i Molecular Systems Biology, 2017.

DOI för In situ genotyping of a pooled strain library after characterizing complex phenotypes Ladda ner fulltext (pdf) av In situ genotyping of a pooled strain library after characterizing complex phenotypes

Kinetics of dCas9 Target Search in Escherichia Coli

Jones, Daniel; Unoson, Cecilia; Leroy, Prune; Curic, Vladimir et al.

Ingår i Biophysical Journal, s. 314A-314A, 2017.

Kinetics of dCas9 target search in Escherichia coli

Jones, Daniel; Leroy, Prune; Unoson, Cecilia; Fange, David et al.

Ingår i Science, s. 1420-1423, 2017.

DOI för Kinetics of dCas9 target search in Escherichia coli

Measuring the Orientation of Single Proteins Interacting with DNA using Fluorescence Polarization Microscopy

Marklund, Emil; Amselem, Elias; Kipper, Kalle; Johansson, Magnus et al.

Ingår i Biophysical Journal, s. 169A-169A, 2017.

Mechanistic Insights into Autoinhibition of the Oncogenic Chromatin Remodeler ALC1

Lehmann, Laura C.; Hewitt, Graeme; Aibara, Shintaro; Leitner, Alexander et al.

Ingår i Molecular Cell, s. 847-859.e7, 2017.

DOI för Mechanistic Insights into Autoinhibition of the Oncogenic Chromatin Remodeler ALC1 Ladda ner fulltext (pdf) av Mechanistic Insights into Autoinhibition of the Oncogenic Chromatin Remodeler ALC1

Nanometer resolution imaging and tracking of fluorescent molecules with minimal photon fluxes

Balzarotti, Francisco; Eilers, Yvan; Gwosch, Klaus C.; Gynnå, Arvid H. et al.

Ingår i Science, s. 606-612, 2017.

DOI för Nanometer resolution imaging and tracking of fluorescent molecules with minimal photon fluxes

Pointwise error estimates in localization microscopy

Lindén, Martin; Curic, Vladimir; Amselem, Elias; Elf, Johan

Ingår i Nature Communications, 2017.

DOI för Pointwise error estimates in localization microscopy Ladda ner fulltext (pdf) av Pointwise error estimates in localization microscopy

Real- Time Single Protein Tracking with Polarization Readout using a Confocal Microscope

Amselem, Elias; Marklund, Emil; Kipper, Kalle; Johansson, Magnus et al.

Ingår i Biophysical Journal, s. 295A-295A, 2017.

Somatic PRDM2 c.4467delA mutations in colorectal cancers control histone methylation and tumor growth

Pandzic, Tatjana; Rendo, Verónica; Lim, Jinyeong; Larsson, Chatarina et al.

Ingår i OncoTarget, s. 98646-98659, 2017.

DOI för Somatic PRDM2 c.4467delA mutations in colorectal cancers control histone methylation and tumor growth

Variation in Mutational Robustness between Different Proteins and the Predictability of Fitness Effects

Lind, Peter A.; Arvidsson, Lars; Berg, Otto; Andersson, Dan I.

Ingår i Molecular biology and evolution, s. 408-418, 2017.

DOI för Variation in Mutational Robustness between Different Proteins and the Predictability of Fitness Effects

Curvature Sensing by a Viral Scission Protein

Martyna, Agnieszka; Gomez-Llobregat, Jordi; Lindén, Martin; Rossman, Jeremy S.

Ingår i Biochemistry, s. 3493-3496, 2016.

DOI för Curvature Sensing by a Viral Scission Protein

Hypothesis: Homologous Recombination Depends on Parallel Search

Elf, Johan

Ingår i CELL SYSTEMS, s. 325-327, 2016.

DOI för Hypothesis: Homologous Recombination Depends on Parallel Search

In Vivo Measurements of Protein Synthesis Kinetics using Single-Molecule Tracking of E.Coli tRNAS

Volkov, Ivan; Aguirre, Javier; Lindén, Martin; Elf, Johan et al.

Ingår i Biophysical Journal, s. 351A-351A, 2016.

Quantification of transcription factor-DNA binding affinity in a living cell

Belikov, Sergey; Berg, Otto G.; Wrange, Orjan

Ingår i Nucleic Acids Research, s. 3045-3058, 2016.

DOI för Quantification of transcription factor-DNA binding affinity in a living cell Ladda ner fulltext (pdf) av Quantification of transcription factor-DNA binding affinity in a living cell

Simulated single molecule microscopy with SMeagol

Lindén, Martin; Ćurić, Vladimir; Boucharin, Alexis; Fange, David et al.

Ingår i Bioinformatics, s. 2394-2395, 2016.

DOI för Simulated single molecule microscopy with SMeagol Ladda ner fulltext (pdf) av Simulated single molecule microscopy with SMeagol

The Chd1 chromatin remodeler shifts hexasomes unidirectionally

Levendosky, Robert F.; Sabantsev, Anton; Deindl, Sebastian; Bowman, Gregory D.

Ingår i eLIFE, 2016.

DOI för The Chd1 chromatin remodeler shifts hexasomes unidirectionally Ladda ner fulltext (pdf) av The Chd1 chromatin remodeler shifts hexasomes unidirectionally

The helical structure of DNA facilitates binding

Berg, Otto G.; Mahmutovic, Anel; Marklund, Emil; Elf, Johan

Ingår i Journal of Physics A, 2016.

DOI för The helical structure of DNA facilitates binding

The Synchronization of Replication and Division Cycles in Individual E. coli Cells

Walldén, Mats; Fange, David; Lundius, Ebba Gregorsson; Baltekin, Özden et al.

Ingår i Cell, s. 729-739, 2016.

DOI för The Synchronization of Replication and Division Cycles in Individual E. coli Cells